Platelet activation was assessed by measuring changes in platelet surface expression of CD40L and CD62P and changes in the number of platelet-leukocyte conjugates relative to the baseline values of each exposure.
There were no significant UFP effects on soluble C-reactive protein (CRP) (Figure 2D), E-selectin, L-selectin, CD62P, intercellular cell adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), serum amyloid A (SAA), or the coagulation markers (D-dimer, Factors VII and IX) [see Supplemental Material, Table 6 (doi:10.
Fifty-four people diagnosed with CFS or fibromyalgia and 23 controls were given five tests to evaluate their blood: fibrinogen (FIB), prothrombin fragment 1 + 2 (F1 + 2), thrombin/antithrombin complexes (T/AT), soluble fibrin monomer (SFM), and platelet activation by flow cytometry (PA) using CD62P
Upregulation of adhesion molecules CD11b on neutrophils and CD62P
on platelets was also significantly inhibited by TNX-224, which is consistent with the inhibition of the release of neutrophil-specific myeloperoxidase and elastase and platelet-specific thrombospondin.
Results for CD62P and CD154 were expressed as percentage of positive events and MFI gated on [CD61.
This goal was met for all but the percentage gated for CD62P and CD154, with estimated CVs of 20% and 26%, respectively.
Effect of glycoprotein Ilb/Illa inhibitors on CD62p
expression, platelet aggregates, and microparticles in vitro.
FITC anti-human CD62P was purchased from BD Biosciences Pharmingen, and peroxidase-conjugated goat anti-mouse IgG was purchased from Chemicon.
Washed platelets (20 [micro]L) stimulated with ADP, U46619, or thrombin were stained with 10 [micro]L of FITC-conjugated anti-human CD62P and, after a 15-min incubation in the dark, were diluted with 1.
MEASUREMENT OF PERCENTAGES OF PLATELETS EXPRESSING CD62P AND OF LEUKOCYTES WITH PLATELETS ATTACHED (PLATELET-LEUKOCYTE AGGREGATES)
For the analysis of CD62P expression, data were acquired in real time with a primary gate set on a dual-parameter histogram of forward light scatter logarithmic scale (abscissa) and side light scatter logarithmic scale (ordinate).
Because shape change is a reversible process (15), CD62P
and CD63 binding is better suited to the detection of activated platelets in vivo.