CD62p

CD62p

a type I transmembrane protein present on activated platelets, endothelial cells, and megakaryocytes that facilitates adhesion of monocytes and neutrophils to activated platelets and to endothelial cells.
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Platelet activation was assessed by measuring changes in platelet surface expression of CD40L and CD62P and changes in the number of platelet-leukocyte conjugates relative to the baseline values of each exposure.
There were no significant UFP effects on soluble C-reactive protein (CRP) (Figure 2D), E-selectin, L-selectin, CD62P, intercellular cell adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), serum amyloid A (SAA), or the coagulation markers (D-dimer, Factors VII and IX) [see Supplemental Material, Table 6 (doi:10.
Fifty-four people diagnosed with CFS or fibromyalgia and 23 controls were given five tests to evaluate their blood: fibrinogen (FIB), prothrombin fragment 1 + 2 (F1 + 2), thrombin/antithrombin complexes (T/AT), soluble fibrin monomer (SFM), and platelet activation by flow cytometry (PA) using CD62P and ADP.
Upregulation of adhesion molecules CD11b on neutrophils and CD62P on platelets was also significantly inhibited by TNX-224, which is consistent with the inhibition of the release of neutrophil-specific myeloperoxidase and elastase and platelet-specific thrombospondin.
Results for CD62P and CD154 were expressed as percentage of positive events and MFI gated on [CD61.
This goal was met for all but the percentage gated for CD62P and CD154, with estimated CVs of 20% and 26%, respectively.
Effect of glycoprotein Ilb/Illa inhibitors on CD62p expression, platelet aggregates, and microparticles in vitro.
FITC anti-human CD62P was purchased from BD Biosciences Pharmingen, and peroxidase-conjugated goat anti-mouse IgG was purchased from Chemicon.
Washed platelets (20 [micro]L) stimulated with ADP, U46619, or thrombin were stained with 10 [micro]L of FITC-conjugated anti-human CD62P and, after a 15-min incubation in the dark, were diluted with 1.
MEASUREMENT OF PERCENTAGES OF PLATELETS EXPRESSING CD62P AND OF LEUKOCYTES WITH PLATELETS ATTACHED (PLATELET-LEUKOCYTE AGGREGATES)
For the analysis of CD62P expression, data were acquired in real time with a primary gate set on a dual-parameter histogram of forward light scatter logarithmic scale (abscissa) and side light scatter logarithmic scale (ordinate).
Because shape change is a reversible process (15), CD62P and CD63 binding is better suited to the detection of activated platelets in vivo.