CD62e

CD62e

a type I transmembrane protein present on endothelium; facilitates adhesion of neutrophils, monocytes, and some T cells to vascular endothelium; enhanced expression occurs at sites of chronic inflammation.
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1% glutaraldehyde in PBS and then incubated with 300 ng/ml CD62E mouse anti human antibody (R&D Systems, Minneapolis, USA) and horseradish peroxidase-linked sheep anti mouse IgG (GE Healthcare, Waukesha, USA).
A pstI polymorphism detects the mutation of serine128 to arginine in CD62E gene- a risk factor for coronary artery disease.
Plasma concentrations of sE-selectin were determined using a CD62E enzyme-linked immunosorbent assay kit (Diaclone Research, Besancon, France).