Immunohistochemical test showed the following: CK (−), vimentin (+), Ki-67 (almost negative), HMB45 (−), CD34 (−), CD31 (−), FCL-1 (−), actin (−), desmin (−), S-100 (+), CD56
(+), Syn (+), and CgA (+) [Figure 1]c.
Seven cases were positive for cytokeratin, 5 for chromogranin, 2 with CD56
and one each with synaptophysin and neuron specific Enolase.
Flow cytometry confirmed the presence of myeloid blasts, which were positive for CD13, CD33, CD34, CD117, HLA-DR, CD56
, and CD64; and negative for CD3 and CD19.
However, it is common for acute leukemias to aberrantly express protein markers more typically associated with other lineages, for example, expression of the myeloid markers CD13 and CD33 in B-ALL or T-ALL and expression of the T/NK-cell markers CD7 and CD56
Neuron specific enolase (NSE), CD56
, vimentin, progesterone receptor (PR) and [beta] catenin were performed on 8,5,3,13 and 16 cases respectively and showed positivity in majority of cases.
Enhancement of peripheral blood CD56
(dim) cell and NK cell cytotoxicity in women with recurrent spontaneous abortion or in vitro fertilization failure.
Immunohistochemical studies were carried out on the cell block and core biopsy sections using antibodies TTF-1 (1:200; Leica), thyroglobulin (1:1000; Dako), ER (1:50; Dako), PR (premade; Leica), GATA3 (1:200; Cell Marque), GCDFP-15 (1:500; Leica), CD56
(Premade; Leica), synaptophysin (1:200; Dako), chromogranin (1:200; Cell Marque), pAx8 (1:100; Cell Marque), villin (1:100; Leica), CK 7 (1:400; Dako) and CK20 (1:200; Novocastra).
Lo siguio el marcador CD56
o NCAM, una molecula de adhesion multivalente que se expresa en las celulas NK, las celulas T activadas, las endocrinas y tejidos cerebrales especificos.
The immunohistochemical stains performed were Neuron Specific Enolase (NSE) (Dako,1:400), Chromogranin A (Dako, 1:5000), CD56
(NovoCas- tra,1:50), Neurofilament (Dako,1:400), S-100 (Dako, 1:1000), Synaptophysin (Biogenex, 1:400), Vimentin (Vim 3B4 Dako, 1:100), CD 99 (mic-2 12E7 Dako, Pre-diluted), Cytokeratin (clone AE1/AE3 Dako 1:50), Epithelial Membrane Antigen (EMA) (E29, Dako, 1:50), Cytokeratin 7 (OV-TL 12/30, Dako, 1:100), CD117 (c-kit - Dako, 1:100), Desmin (D33, Dako, 1:150), Cytokeratin CAM 5.
Immunohistochemical examination of the specimen was positive for cytokeratin (CK)18, CK19, CK5/6 and CD5 and CD117, and negative for CK7, TTF1, CD56
and CD20 markers (Figure 4).
On immunohistochemistry, Ki67 was positive in tumor cells (70-80%), synaptophysin was positive in tumor cells, CD56
was positive in tumor cells and chromogranin was weak focal positive.
Immunohistochemical staining demonstrated consistent expressions of epithelial membrane antigen (EMA), progesterone receptors (PR), and CD56
in the tumor cells.