1% BSA in PBS, and incubated with an Alexa Fluor 488 1:100 conjugated antibody against the CD49f surface marker (Biolegend) on ice 1h.
After FACS-sorted for CD49f selection, the enriched fraction of fresh cell was homogenous, containing cell with similar sizes and shapes (Figure-1C).
19 In the present study, we isolated CD49f (a6 integrin) cells using LIF, EGF, GDNF and collagen-coated dish to improve the survival and proliferation of cells in vitro.
CD105, CD44, CD29, 9 (CD9), CD49f
, CD90, major histocompatibility complex class I (MHC I), multipotent markers octamer-binding transcription factor 4 (Oct-4; the main regulator of differentiation in pluripotent cell line), stage-specific embryonic antigen 4 (SSEA-4; the marker used to differentiate between human and mouse cells and used to characterize pluripotent stem cells), and stromal-derived factor-1 (STRO1; used as identification, isolation, and functional testing of human bone marrow stromal progenitor cells) have been identified under fluorescent microscopy or by flow cytometry.
2] the levels of the adhesion molecules CD49f
, CD49b, CD29, and CD44 were increased.
Machida and colleagues (2012) also found that TISCs isolated from the livers of alcohol-fed HCV Ns5a transgenic mice and from alcoholic patients carried molecules called CD133 and CD49f (i.
CD49f enhances multipotency and maintains stemness through the direct regulation of OCT4 and SOX2.
Los antigenos especificos para celulas condrogenicas como CD49c, CD49e, CD49f
y CD151 pueden ser evaluados mediante citometria de flujo, segun Grogan et al (21) su expresion indica alta capacidad condrogenica in vitro.