Fifty [micro]l of EDTA blood was incubated with 5 [micro]l anti CD31 monoclonal antibody labeled with fluorescinisothiocyanate (FITC) (Bekhman Coulter, USA) and 5 [micro]l anti CD42b
monoclonal antibody labeled with phycoerythrin (PE) (Bekhman Coulter, USA) for 30 min.
1] IgG1, [kappa] (clone WM59, BioLegend)) and 3 [micro]L PerCP-conjugated anti-human CD42b (400 [micro]g [mL.
CD31 is expressed on both platelets and endothelial cells, whereas CD42b is restricted to platelets, allowing discrimination between PMVs and EMVs (colored blue).
Other mechanisms described in the literature include increased activation of Glycoprotein IIb/IIIa, CD41a, CD42b
, CD62P and CD6 (3,2,6) prothrombin fragment F1+ 2, thrombin-antithrombin III complex and fibrinopeptide A.
Sections of bone marrow core biopsies or clot sections were stained with CD34 (prediluted with no antigen retrieval; clone QBEnd/10, Ventana, Tucson, Arizona), CD20 (1:200 dilution with CC1 antigen retrieval method; clone L26, Dako, Carpinteria, California), CD3 (prediluted with CC1 antigen retrieval method; clone 2GV6, Ventana), myeloperoxidase (polyclonal, prediluted with no antigen retrieval; Cell Marque, Rocklin, California), hemoglobin A (polyclonal, 1:3000 dilution with no antigen retrieval; Dako), CD68 (1:50 dilution with CC1 antigen retrieval method; clone PG-M1, Dako), and CD42b
(1:200 dilution with CC1 antigen retrieval method; clone MM2-174, Novocastra, Newcastle upon Tyne, United Kingdom).
5 mg/mL) 45 Platelet function- related markers, % CD41 (GPIIb) 0 CD61 (GPIIIa) 0 CD42b
100 Analyte Day 3 Reference interval WBC, x [10.
3,9,10) One such antigen, CD42b
(GPIb[alpha]), is part of the CD42a-d complex that serves as a receptor for von Willebrand factor and thrombin.
2) The blast phenotype in TMD is variable, but the immature cells have usually been positive for platelet-specific antigens CD41, CD42b
, and CD61, for panmyeloid antigens in some cases, frequently for the stem cell marker CD34, and often coexpressed CD7.
The BM aspirates or cell suspensions of the BM core biopsy were analyzed on a flow cytometer (Cytoronabsolute, Ortho Diagnostic Systems, Raritan, NJ) for various antigens using standard techniques and the following commercially available monoclonal antibodies: CD1, CD15, HLA-DR (Ortho Diagnostic); CD2, CD10, CD13, CD14, CD19, CD20, CD24, CD33, CD34, CD56, CD64, CD117, and terminal deoxynucleotidyl transferase (TdT) (Coulter Immunology, Hialeah, Fla); CD3, CD4, CD7, and CD45 (Becton-Dickinson, San Jose, Calif); CD5 and CD8 (Gen Trak, Inc, Wayne, Pa); CD41 and CD42b
(Dako Corporation, Carpinteria, Calif); [Kappa] and [Lambda] light chains (Kallestad Inc, Chaska, Mass); and intracytoplasmic CD3/MPO (cCD3/MPO) (Caltag, Burlingame, Calif).