Buy supplies for the isolation of CD34
on the appliance Clinimacs plus.
Utility of immunohistochemical staining with FLI1, D2-40, CD31, and CD34
in the diagnosis of acquired immunodeficiency syndrome-related and non-acquired immunodeficiency syndrome-related Kaposi sarcoma.
They selected only those cells that bore a membrane protein called CD34
-- a marker for early stem cells -- but lacked another membrane protein called major histocompatibility complex class II, which the donor cells could use to recognize and attack their new host.
is a marker of lymphatic and vascular endothelium.
Staining with CD34
highlighted endothelial lining in 12 of 22 (55%) cases.
Subsequent immunohistochemistry confirmed the diagnosis of a fibromyxomatous spindle-cell neoplasm, not further classified, with an unusual immunophenotype containing vimentin, S-100 protein, and desmin and negative for muscle-specific actin (MSA), smooth-muscle actin (SMA), CD31, and CD34
Dick, a stem-cell researcher a the University of Toronto, notes that other researchers have already shown that cells bearing CD34
but lacking Lin can grow for months among marrow connective-tissue cells in the laboratory.
Monoclonal antibodies directed against CD31 (platelet/ endothelial cell adhesion molecule, PECAM1) and CD34
(a hematopoietic progenitor cell surface protein) are relatively sensitive and specific markers of endothelial differentiation that are expressed in the majority of vascular tumors.
Yet, the mechanism by which the infusion of CD34
stem cells improves liver function remains elusive, they said.
The tumor cells stained strongly positive for CD34
Because of their CD34
positivity and the presence of spindle-shaped and stellate cells embedded in a vascular, basophilic matrix, SAFMs can be confused with superficial angiomyxomas.
Magnetic cell selection by technical INF gamma or sorting CD34