With suspicion of LAD it was decided to investigate it and diagnosis was confirmed by flow cytometry showing absence of CD18, CD11b, CD11c
The monoclonal antibodies (MoAb) used for diagnosis of AL were: for B-lineage: CD19, CD22, CD20, CD10, for T-lineage: CD2, CD3, CD5, CD7, CD4, CD8, for myelo-monocytic lineage: CD13, CD33, CD11c
, CD64, CD14, other cells surface markers: CD34, CD117, HLA-DR and intracellular MoAb: CD79a, CD3 and MPO.
World Health Organization 2008/2016 Criteria for Mixed-Phenotype Blasts (a) Lineage Markers Myeloid MPO (flow cytometry, immunohistochemistry, or enzyme cytochemistry) -OR- Monocytic differentiation (at least 2 of the following: NSE cytochemistry, CD11c
, CD14, CD64, lysozyme) T lineage Strong (b) cytoplasmic CD3 -OR- Surface CD3 B lineage Strong (b) CD19 with at least 1 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 -OR- Weak CD19 with at least 2 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 Abbreviations: MPO, myeloperoxidase, NSE, nonspecific esterase.
(eBioscience, San Diego, CA, USA) by using methods described previously (18).
2008 WHO Diagnostic Criteria for MPAL  Lineage Markers Myeloid Myeloperoxidase or Monocytic differentiation (At least two of the following: NSE, CD11c
, CD14, CD64, lysozyme) T Lineage Cytoplasmic CD3 or Surface CD3 B Lineage Strong CD19 and at least one of the following with strong expression: CD79a, cytoplasmic CD22 or CD10 OR Weak CD19 and at least two of the following with strong expression: CD79a, cytoplasmic CD22 or CD10 Table 2.
In all cases, an immunophenotypic analysis was performed by flow cytometry, including CD19, CD5, CD22, CD23, CD38, CD25, CD103, CD11c
, FMC7, BCL2, CD10, CD20, and surface immunoglobulins ?
Three cell subsets were identified based on the expression of CD11c
and Ly-6G (right panels), and the overlaid histogram of MHC-II on these cells is shown.
The remaining cells were then labelled with antibodies specific for CD11c
and MHC class II  and MHC class IIhiCD11cmed cells sorted by FACS using the FACSAria (BD Biosciences).
In addition, vanadium can also modify immune CD11c
and MHC-II expression in thymic dendritic cells by decreasing the presence of CD11c
surface marker on mouse thymic dendritic cells as a result of vanadium pentoxide ([V.
We also investigated the surface marker CD11c
and the costimulatory molecules of DCs during treatment with RS extract.
The microglia express a molecule, CD11c
, on their surface.
Marcadores de inmunoperoxidasas para CD 20 que muestra reactividad difusa con expresion BCL2 y CD11c