T lymphocytes in lung were analyzed by measuring cell adhesion molecule CD11a
For the EV Array, the following antibodies were used for capturing: CD11a
(HI111) from Ab Biotech (San Diego, CA, USA); Flotillin-1 and TSG101 (5B7) from Abcam (Cambridge, MA, USA); CD3 (HIT3a), CD14 (M5E2), CD16 (3G8), CD28 (L293), CD49d (L25), and CD56 (3G8) from BD Biosciences (Mountain View, CA, USA); CD41 (HIP8), CD63 (MEM-259), HLA-ABC (W6/32), and Alix (3A9) from Biolegend (San Diego, CA, USA); ICAM-1 (R6.
The following antibodies were used as follow: For flow cytometry determination, CD3, CD8, CD54 (intercellular adhesion molecule [ICAM]-1), CD106 (vascular adhesion molecule [VCAM]-1), CD11a
(lymphocyte function-associated antigen-1 [LFA-1]), CD29 (integrin [sz]1), IL-27, IFN-a, IL-17, phosphorylated (p)-STAT1 (pY701), p-STAT1 (pS727), p-STAT2 (pY689), p-STAT3 (pS727), p-STAT3 (pY705), p-STAT4 (pY693), p-STAT5 (pY694), p-STAT6 (pY641), TCCR-WSX-1 (IL-27 receptor), IL-17 receptor (R), IFN-aR1, and Ki-67 mAbs were purchased from BD Biosciences (Franklin Lakes, USA), eBioscience (San Diego, USA), or R and D systems (Minneapolis, USA); for cytokine neutralization, IL-27, IFN-a, IL-17, ICAM-1 and VCAM-1 mAbs were from eBioscience.
Efalizumab is a recombinant humanised monoclonal IgG1 antibody that binds to CD11a
, a subunit of leukocyte function-associated antigen 1 (LFA-1) (24).
These cells are characterized by the surface pattern of effector-type or effector-memory T cells (expression of CD45RA, CD11a
, CD11b, CLA, [alpha]E[beta]7 and absence of CD27, CD28, CD56, CD62L).
17] found lower mean CD3+, CD4+, CD18 and CD11a
and CD4+/CD8+ ratio in 20 preterm and term neonates with sepsis, compared with 23 healthy control subjects.
Intravascular large B-cell lymphoma cells have been demonstrated to lack cell surface proteins critical to lymphocyte transvascular migration, including CD29 ([beta]1 integrin) and CD54 (ICAM-1 or CD11a
, B lenfositleri, monosit, makrofaj gibi hucrelerin yuzeyinde bulundugu icin, ajan T hucreler disinda diger hucrelerinde aktivasyon, migrasyon ve fonksiyonlarini bozmaktadir.
However, this is not related to CC chemokine receptor 4, cutaneous lymphocyte-associated antigen, or CD11a
expression by CD4 (+) T cells or their physical capacity for migration.
La hidralacina, procainamida y la luz ultravioleta producen en la celula T la disminucion de la metilacion, incrementan la expresion de CD11a
e inducen auterreactividad in vitro.
Lenfosit fonksiyonu ile iliskili antijen-1 (LFA-1) yapisindaki CD11a
kismina baglanarak T lenfositlerin, antijen sunan hucreler, vaskuler endotelyal hucreler ve keratinositlerde eksprese edilen interseluler adezyon molekulu-1 (ICAM-1) ile etkilesimini engeller (3,4).
Efalizumab: es un anticuerpo monoclonal IgG1 humanizado dirigido contra la subunidad CD11a
del LFA-1, que perturba la presentacion antigenica y la activacion y proliferacion celulares.