Antibodies to myeloperoxidase, lysozyme, CD68, CD45, CD34 and CD117
are all useful IHC stains for the evaluation of myeloid sarcoma7.
CD3, CD4, CD9, CD11, CD19, CD20, CD22, CD26, CD27, CD28, CD29, CD33, CD37, CD40, CD44, CD45, CD47, CD52u CD55, CD56, CD66, CD70, CD74, CD80, CD95, CD98, CD100, CD117
, CD135, CD137, CD152, CD200, CD223, CD227, CD246, CD248, CD274, CD276, CD279
Immunohistochemistry is not usually of value in distinguishing between salivary gland tumors, although differential expressions of cytokeratins, S-100 protein, GFAP, CD117
, MCM2 (minichromosome maintenance proteins), and MYB may be of help.
The tumor cells were immunohistochemically positive for CK7, CD117
, and Hale's colloidal iron.
These immunohistochemical studies included reagents specific for CD3, CD11c, CD20, CD33, CD43, CD68, CD117
, CD163, lysozyme, and myeloperoxidase.
Neuropilin-1 mRNA level by real-time PCR showed a positive significant correlation with peripheral blood blast percentage and CD117
and a negative correlation with hemoglobin level in de novo AML patients.
a) Gene class Gene Selected exons Signaling BRAF 11, 15 CBL 8, 9 ETV6 Complete FLT3 20 JAK2 12, 14 KIT 8, 17 KRAS 2, 3 SH2B3 2 MPL 10 NRAS 2, 3 Transcription CEBPA Complete NPM1 11 RUNX1 Complete TP53 Complete WT1 7, 9 Epigenetics ASXL1 12 DNMT3A Complete EZH2 Complete IDH1 4 IDH2 4 TET2 Complete KDM6A Complete RNA splicing SF3B1 12, 13, 14, 15, 16 SRSF2 1, 2 U2AF1 2, 6 ZRSR2 Complete (a) BRAF, v-raf murine sarcoma viral oncogene homolog B1 (synonym: BRAF1); ETV6, ets variant 6 (synonym: TEL); KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (synonyms: c-Kit, CD117
, SCFR); SH2B3, SH2B adaptor protein 3 (synonyms: IDDM20, LNK); WT1, Wilms tumor 1 (synonyms: AWT1, WAGR, ).
RT-PCR primers for pluripotency (Oct3/4, Nanog, Sox2), MSC surface markers (CD4, CD8A, CD25, CD33, CD34, CD44, CD54, CD61, CD80, CD105, CD117
, Flk-1 and HMGA2) and GAPDH internal control were shown in
CD34 plus CD117
to identify myeloid progenitor cell populations) in conjunction with antibodies to identify specific intracellular signaling molecule activation.
There was partial dim expression of CD117
and CD4, and no CD56 expression.
Kromozom 4q12'de yerlesmis, C-kit veya CD117
geni olarak da bilinen KIT proto-onkojenindeki mutasyon sonucu olusur (15).
Pathological analysis revealed a malignant spindle cell tumor with negative immunohistochemical staining for CD117
and CD10 and mild reactivity for desmin and muscle specific actin, making low grade leiomyosarcoma the most likely diagnosis.