These cells expressed CD90, CD44, CD105
, and CD73 but not CD45, CD11b, CD19, HLA-DR, and CD34 [Figure 2]b.
Es por esto que diversos investigadores han cuantificado vasos sanguineos mediante tecnica de inmunohistoquimica contra CD31, CD34, y CD105
(Cheng et al, 2014; Andisheh-Tadbir et al.
Third-passage DFSCs were incubated with the antibodies of positive markers for MSCs of human CD29 allophycocyanin (APC), CD73 phycoerythrin (PE), CD105
PE, and CD146 fluorescein isothiocyanate (FITC) and with the antibodies of negative markers of CD14 PE, CD34 FITC, CD45 APC, and HLA-DR PE (BD Biosciences, USA) for 15 min at room temperature in the dark.
PgLPS promoted cell proliferation, produced IL-1[beta] and TNF-[alpha], and did not affect the expression of stem cell markers, STRO-1, CD105
MSCs are characterized on the basis of surface expression markers as CD73, CD90, and CD105
positive, and CD34 and CD45 negative .
2005), CD73, CD90 and CD105
(DE MATTOS CARVALHO et al.
Elevated plasma CD105
and vitreous VEGF levels in diabetic retinopathy.
The criteria of the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy reported that stem cells that expressing the markers of CD44, CD90, CD73 and CD105
are considered mesenchymal and those that express markers of CD19, CD14, CD34 or CD45 are regarded hematopoietic stem cells.
5] rat ADSCs were suspended in 50 [micro]L PBS with anti-human CD90 or anti-human CD105
conjugated with either phycoerythrin or fluorescein isothiocyanate (BD Biosciences, Franklin Lakes, NJ, U.
, CD44, CD29, 9 (CD9), CD49f, CD90, major histocompatibility complex class I (MHC I), multipotent markers octamer-binding transcription factor 4 (Oct-4; the main regulator of differentiation in pluripotent cell line), stage-specific embryonic antigen 4 (SSEA-4; the marker used to differentiate between human and mouse cells and used to characterize pluripotent stem cells), and stromal-derived factor-1 (STRO1; used as identification, isolation, and functional testing of human bone marrow stromal progenitor cells) have been identified under fluorescent microscopy or by flow cytometry.
Voinea, "VEGF and CD105
immunoexpression in squamous cervical carcinomas and associated precancerous lesions," Romanian Journal of Morphology and Embryology, vol.
In addition, we also examined whether Dx modulated expression of CD molecules, such as CD105
, CD137, and CD166, which are associated with atherosclerosis.