Flow cytometry showed a 96% variable size, clonal B cell population expressing CD10, CD11c, CD19, CD20, CD22, CD45, CD52, CD103
, CD123, BCL2, and Kappa.
Flow cytometric analysis exhibited [lambda] monoclonal B cells with coexpression of CD10 and FMC-7, which were negative for CD5, CD23, CD11c, CD25, CD103
, and [kappa] light chain.
antibody was purchased from BioLegend (San Diego, CA).
The immunophenotype differs somewhat from classical HCL in that there is variable expression of CD103
and lack of CD25 .
today announced a strategic partnership with Biogen Idec to develop therapies targeting CD103
for the treatment of chronic inflammatory diseases including potential respiratory, gastrointestinal, and autoimmune indications.
Diagnosis CD5 CD10 CD19 CD20 CD23 SLL/CLL (a) + - + +(w) + Mantle cell lymphoma + - + + - Follicle center lymphoma - + + + -/+ Marginal zone lymphoma - - + + - Hairy cell leukemia - - + + - Diagnosis CD79b FMC-7 CD25 CD11C CD103
SLL/CLL (a) - - -/+ +/_ - Mantle cell lymphoma + + - - - Follicle center lymphoma +/- +/- - - - Marginal zone lymphoma +/- +/- -/+ + - Hairy cell leukemia +/- +/- +/- + + (a) SLL/CLL, small lymphocytic lymphoma/chronic lymphocytic leukemia; +, positive; -, negative; +/-, often positive; -/+, occasionally positive; w, weak expression.
Hairy cells are also characteristically CD11c, CD19, CD20, CD22, CD25, CD103
, and variably CD38 positive.
They are associated with a highly specific intestinal IEL population that has a normal cytologic appearance and surface CD103
expression but has an aberrant immunophenotype, characterized by the expression of intracellular CD3[epsilon] without the usual surface T-cell markers (CD3, CD8, CD4, and TCR).
Analysis of the duodenal biopsy with a 4-color flow cytometer (Cytomics FC500, Beckman Coulter, Fullerton, Calif) demonstrated an abnormal T-cell population that expressed 99% CD2, 4% CD3, 100% cytoplasmic CD3, 4% CD4, 2% CD5, 99% CD7, 98% CD8, 2% CD16, 2% CD25, 98% CD56, 2% CD57, and 94% CD103
positive cells (Figure 2, A through F).
41) Hairy cell leukemia is best characterized by the use of flow cytometry with a distinctive B-cell immunophenotype including expression of CD103
, CD25, and CD11c.
These cells were negative for CD5, CD10, CD23, CD11C, and CD103
Immunotech) (Figure 2, C), and did not express CD103
(2G5, Immunotech SA) (Figure 2, D).