CAT Assay

Chloramphenicol Acetyl Transferase Assay. A technique used to determine whether a given segment of DNA has an attached promoter, which would up-regulate transcription
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Activity of CAT, GSH-Px and SOD, as well as concentration of MDA were assayed by using CAT assay kit, GSH-Px assay kit, SOD assay kit and MDA assay kit, respectively.
By these modifications of the plasma CAT assay, we acceptably reduced quenching and issues related to unequal distribution of red blood cells.
This aspect, coupled with the radioactivity involved in the CAT assay, taints the CAT gene's usefulness as a reporter gene, says Keith V.
The CAT assay has been evaluated in studies including comparisons with the BQ procedure, but not in multicenter evaluations.
LDL prepared by ultracentrifugation was measured by the CAT assay and revealed a mean recovery for LDL of 95% in 31 samples (108).
The very limited evaluation suggests that the CAT assay seems to be relatively specific for LDL-C but that some VLDL-C is measured as LDL-C, indicating interference with high TGs.