CASP3


Also found in: Acronyms.

CASP3

A gene on chromosome 4q34 that encodes a so-called effector caspase belonging to the cysteine-aspartic acid protease (caspase) family which, once activated by proteolytic processing, plays a central role in the execution phase of apoptosis, as well as various stages of embryological development. CASP3 is highly expressed in the lung, spleen, heart, liver and kidney.
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Following treatment with different concentrations of dioscin for 24 h, the protein expression levels of TP53, BAX, BCL2 and CASP3 (P17) were detected by Western blotting.
The protein expression of TP53, BAX and CASP3 protein were evaluated by western blot analysis.
CASP3 is a major mediator of apoptotic and necrotic cell death (Behar et al.
B-Cell CLL/Lymphoma 2; CASP3, Caspase 3; 5-FU, 5-Fluorouracil; RPMI-1640.
With respect to the genes we have analyzed, the SD increases with normalization for IFNG in liver and for CASP3 in blood.
Sample types and genes Liver ACTB IL1B CASP3 FGF7 Mean Cq 20.
Another study showed that capsaicin stimulated apoptosis of Swiss albino mice via CASP3 induction (Anandakumar et al.
In conclusion, capsaicin induced apoptosis by activating CASP3 and BAX, and it decreased mRNA expression of adipogenesis-related genes such as PPARG, CEBPA, FABP4, and SCD.
Hesperidin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 4,6-diamidino-2-phenylindole (DAPI), 3,3'-diaminobenzidine (DAB), and CASP3 assay kits were purchased from Sigma-Aldrich Chemical Co.
PCR was performed using the following primers: for BAX, 5'-AAC ATG GAG CTG CAG AGG ATG ATT-3' and 5'-CTG GTC TTG GAT CCA GCC AGC CCA ACA G-3'; for BCL2, 5'-CCT CTG TTT GAT TTC TCC TGG CTG T-3' and 5'-TGG AAA GCG AAT CTA TGT TTA CAG G-3'; and for CASP3, 5'-ATC TCG GTC TGG TAC AGA TGT CGA T-3' and 5'-TGA ATT TCG CCA AGA ATA ATACCA-3'.
CASP3 activity was measured using an assay kit according to the manufacturer's protocol.
Mouse pro-CASP3 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and mouse active CASP3 antibody (BD Biosciences, NJ, USA) were used as primary antibodies.