CASP3


Also found in: Acronyms.

CASP3

A gene on chromosome 4q34 that encodes a so-called effector caspase belonging to the cysteine-aspartic acid protease (caspase) family which, once activated by proteolytic processing, plays a central role in the execution phase of apoptosis, as well as various stages of embryological development. CASP3 is highly expressed in the lung, spleen, heart, liver and kidney.
References in periodicals archive ?
In conclusion, capsaicin induced apoptosis by activating CASP3 and BAX, and it decreased mRNA expression of adipogenesis-related genes such as PPARG, CEBPA, FABP4, and SCD.
Sample types and genes Liver ACTB IL1B CASP3 FGF7 Mean Cq 20.
Hesperidin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 4,6-diamidino-2-phenylindole (DAPI), 3,3'-diaminobenzidine (DAB), and CASP3 assay kits were purchased from Sigma-Aldrich Chemical Co.
PCR was performed using the following primers: for BAX, 5'-AAC ATG GAG CTG CAG AGG ATG ATT-3' and 5'-CTG GTC TTG GAT CCA GCC AGC CCA ACA G-3'; for BCL2, 5'-CCT CTG TTT GAT TTC TCC TGG CTG T-3' and 5'-TGG AAA GCG AAT CTA TGT TTA CAG G-3'; and for CASP3, 5'-ATC TCG GTC TGG TAC AGA TGT CGA T-3' and 5'-TGA ATT TCG CCA AGA ATA ATACCA-3'.
CASP3 activity was measured using an assay kit according to the manufacturer's protocol.
Mouse pro-CASP3 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and mouse active CASP3 antibody (BD Biosciences, NJ, USA) were used as primary antibodies.
Hesperidin induced typical apoptosis at both 10 and 100 [micro]M, with dose-dependent down-regulation of BCL2 expression, and up-regulation of BAX and CASP3 mRNA (Fig.