C-banding

C-band·ing stain

a selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, except for the Y chromosome its long arm usually stains throughout.

C-banding

a technique of chromosomal staining in which chromosomes are exposed to alkaline and then acid conditions, in order to reveal bands of constitutive HETEROCHROMATIN that are identified with Giemsa stain.
References in periodicals archive ?
At least 6 individuals per species were subjected to the C-banding and fluorochrome techniques.
The C-banding technique was performed according to Sumner (1972) with modifications proposed by Barros et al.
Results for C-banding revealed the presence of heterochromatin in the telomeric regions of the short arms: on a large metacentric chromosomal pair and on a sabmetacentric chromosomal pair.
stentina using cytogenetic approaches, such as C-banding, in situ RE banding, FISH with major ribosomal gene (ITS1), and telomere sequence (TTAGGG)n.
This C-banding pattern seems to be characteristic of spiders.
In spermatogonial mitosis, after C-banding, sex chromosomes have shown two different patterns.
The Andean specimens present C-heterochromatic blocks in most of their 22 chromosomes, whereas non-Andean specimens have only 4-7 autosomes with C-banding.
Using flow cytometry for DNA quantification and C-banding technique, we have determined the karyologic changes that have occurred during the dispersal of T.
C-banding analyses and the evolution of heterochromatin among arvicolid rodents.
Jellen, then a graduate student at the University of Minnesota, modified a staining technique called C-banding.
The methodology used for the C-banding was based on that of Sumner (1972) and sequential staining with the fluorochromes [CMA.
Afterwards, the application of differential staining techniques such as Ag-NORs for nucleolar organizer regions, C-banding for heterochromatin or G-banding for individual chromosome identification allowed the identification of specific chromosome pairs in the karyotypes of bivalve species (see Thiriot-Quievreux 2002, for review).