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Bouin's fixative |
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Bouin's fixative a tissue preservative composed of saturated picric acid, formaldehyde and glacial acetic acid. Particularly suitable for trichrome connective tissue stains. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| The crania were dissected free and fixed for 2 h in Bouin's fixative, rinsed in running tap water for 30 min, and then refrigerated overnight. Gonads (from frogs at 1 month postmetamorphosis) attached to kidneys were fixed with Bouin's fixative. Immediately after collection, animals were euthanized in benzocaine, fixed in Bouin's fixative for 48 hr, and preserved in 70% ethanol. |
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