Bouin's fixative

Bouin's fixative

A picric-acid-based compound, which is the fixative of choice for testicular biopsies. It is little used in practice, as it must be removed from tissue before staining with basic dyes and causes DNA methylation, compromising PCR-molecular diagnostics.

Bouin's fixative

a tissue preservative composed of saturated picric acid, formaldehyde and glacial acetic acid. Particularly suitable for trichrome connective tissue stains.
References in periodicals archive ?
Fifteenth days after the start of the study, in sterile conditions, the left and right testes were removed by creating a gap in the lower abdomen, and the left testis was placed in Bouin's fixative solution for histological examination.
The left testis was removed from Bouin's fixative solution and tissue molding with paraffin and by microtome cut sections with a thickness of 5 microns were produced and stained with hematoxylin-eosin method.
Whole fish were fixed in Bouin's fixative and embedded in paraffin, and the gonad region was sectioned to 4 [micro]m, stained, and analyzed by light microscopy.
Gonads (from frogs at 1 month postmetamorphosis) attached to kidneys were fixed with Bouin's fixative.
The uterus was immediately weighed, placed on absorbent cardboard in order to maintain the original in vivo orientation, fixed in cold Bouin's fixative, and processed for histologic and morphometric examination.