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Southern blot |
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Southern blot a technique for detecting specific DNA sequences following agar gel electrophoresis of a set of DNA restriction enzyme digestion fragments. The fragments after electrophoresis are transferred to a nitrocellulose or nylon membrane by applying the membrane to the gel; the membrane is probed with a radioprobe that hybridizes to one or more of the separated fragments and used to produce an autoradiogram. Named for Edward Southern. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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