The Birbeck granule, a trilaminar rod-like organelle with a terminal expansion resembling a tennis racquet, is distinctive.
Electron microscopy was significant for the presence of numerous Birbeck granules (Figure 3).
Langerhans cells have cytoplasmic inclusion bodies known as Birbeck granules
; a definitive diagnosis requires electron microscopy of Birbeck granules
or CD 1 antigenic determinants by immunohistochemistry.
The immunoprofile is (CD1a+, S100+), and some cells should have the characteristic LC features of grooved nuclei and/or Birbeck granules
Langerhans cell histiocytosis is a neoplastic proliferation of Langerhans cells, demonstrating immunohistochemical expression of CD1A and S100 as well as ultrastructural evidence of Birbeck granules
S-100, CD 68 and CD-1a stains were positive and Birbeck granules
were seen on electron microscopy (EM), confirming the diagnosis.
The hallmark of Langerhans cells is the ultrastructural presence of cytoplasmic Birbeck granules
, which are rod- or tennis-racket-shaped structures (figure, D).
6) Birbeck granules
are the characteristic ultrastructural findings in LCs.
15) The sighting of Birbeck granules
(also known as HX bodies) in the cytoplasm of Langerhans' cell histiocytes on electron microscopy is pathognomonic.