Bglii


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Bglii

A type-II homodimeric restriction endonuclease—popularly referred to as bagel two—which cuts DNA in a staggered manner wherever it finds the sequence AGATCT.
References in periodicals archive ?
Southern blot analysis showed an unusual banding pattern with a [zeta]-globin gene probe, with additional smaller bands in BamHI and BglII digests (an EcoRI digest appeared normal).
Two intronic sequence differences (between exons 7 and 8) create unique BglII and AseI restriction sites (3) that can be used to distinguish each allele by restriction fragment length polymorphism assay.
used X-ray crystallography to take molecular pictures of the restriction enzyme BglII in action.
CXCR4 gene was also digested with BglII and Sail and cloned into pBabe-puro retroviral vector.
The annealed oligonucleotides were ligated into the BglII and HindIII sites of the retroviral vector pSuper.
Southern analysis of the DNA of both patients revealed, instead, an unusual ~20-kb BglII band when hybridized with either the a- or [zeta]-globin probe (Fig.
Genome typing by restriction analysis was performed by using 12 restriction enzymes, BamHI, BcII, BgI, BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmaI, XbaI , and XhoI, as previously described (5).
The modified pGL2 vector contains a minimal TATA sequence between BglII and HindIII.
Subsequently, the simian virus 40 (SV40) early promoter was cut out with BglII and HindIII, and replaced with the thymidine kinase (TK) promoter of the pRL-TK vector (Promega) or one of several other promoters (the 3' region of the TK promoter, the cytomegalovirus (CMV) promoter, or the minimal CMV promoter and the 3' region of the CMV promoter (201 and 265 bp)) amplified using the following PCR primers:
Then, the oligonucleotides 5'-GATCTATCGATTCTAGAGGATCCTCGAGATATCCC-3' and 5'-GGGATATCTCGAGGATCCTCTAGAATCGATG A-3' (containing BglII, ClaI, XbaI, BamHI, XhoI, EcoRV) were ligated to this smaI-smaI fragment from pIND/Hygro and then digested with BglII and HindIII (about 300 bp, contains MCS and hs).
The specificity of this assay is enhanced by the use of restriction enzymes, XbaI or BglII, allowing the detection of mutations at the restriction sites for these enzymes.