Ber-Ep4

Ber-Ep4

A monoclonal antibody used in histopathology to differentiate glandular epithelium (usually positive) from mesothelium (usually negative).
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Immunohistochemical studies were performed on 5-[micro]m-thick sections of formalin-fixed, paraffin-embedded tissue, using standard techniques, heat-induced epitope retrieval buffer, and primary antibodies against BerEP4 (clone Ber-EP4, 1:20; Dako, Carpinteria, California), CK14 (clone LL002; Cell Marque, Rocklin, California), and CK17 (clone E3, 1:50; Dako).
Results were negative for carcinoembrionary antigen and the epithelial glycoprotein Ber-EP4, whereas results were positive for the mesothelial markers cytokeratins, calretinin, epithelial membrane antigen, and HBME-1, thus leading to the correct diagnosis of peritoneal epithelial mesothelioma.
Efficiency of Ber-EP4 antibody for isolating circulating epithelial cells before RT-PCR detection.
1) Based on their sensitivity and specificity, calretinin (Figure 11, A and B), CK5 or CK5/6 (Figure 12, A and B), WT-1 (Figure 13, A through C), and D2-40 (podoplanin) (Figure 14, A and B) are the best positive mesothelioma markers; and MOC-31 (Figure 15, A through C), Ber-EP4, carcinoembryonic antigen (CEA), and Lewis(y) antigen blood group 8 (BG8) are the best overall carcinoma markers.
Value of the Ber-EP4 antibody in differentiating epithelial pleural mesothelioma from adenocarcinoma: the M.
2, CK18, and CK7 are positive more frequently than epithelial membrane antigen, carcinoembryonic antigen (CEA), CD15, and Ber-EP4.
The Ber-EP4 antibody is generated from mice immunized with cells from the MCF-7 breast carcinoma cell line and 2 noncovalently bound glycopeptides.
3, Ber-EP4, and pancytokeratin) usually supports an epithelial origin (Figure 4, A and B).
2 Becton Dickinson, San Jose, Calif Epithelial E29 Dakopatts, Glostrup membrane antigen Denmark Calretinin Poly Swant, Bellinzona, Switzerland Human mesothelial HBME-1 Dako cell antigen Human epithelial antigen Ber-EP4 Dako Carcinoembryonic antigen Poly Dako Human pulmonary 44-3A6 Affinity BioReagents, adenocarcinoma antigen Golden, Colo Vimentin V9 Dako S100 4C 4.
Many traditional markers, including carcinoembryonic antigen, Leu-M1, Moc-31, E-cadherin, thyroid transcription factor-1, and Ber-Ep4 are negative in mesotheliomas and positive in adenocarcinomas.
They were negative for epithelial membrane antigen, cytokeratin (high and low molecular weight), CK20, Ber-EP4, breast-2 antibodies, estrogen receptor, progesterone receptor, muscle-specific actin, smooth muscle actin, desmin, HMB-45, S100, neuron-specific enolase, chromogranin, synaptophysin, CD34, leukocyte common antigen, CD3, CD20, CD57, CD68, and factor 13a.