BHK cell

BHK cell

Baby hamster kidney cell. An established diploid cell line descended from a clone (Clone 13) isolated by Stoker and McPherson from a rapidly growing primary culture of newborn Syrian hamster kidney tissue. BHK-21 cells are susceptible to human adenovirus D, reovirus 3 and vesicular stomatitis virus, and used for transformation and transfection. BHK cells are often described as fibroblastic, but also smooth muscle-like in that they express the muscle intermediate filament protein desmin; widely used as a viral host in studies of oncogenic transformation and cell physiology.
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BHK cell expression showed that the transporter was located in the cell membrane.
Transient expression of porcine SLC7A7 in BHK cells
coli and then transfected into the BHK cells using Lipfectin 2000 (Invitrogen) following the manufacturer's protocol for the expressional localization of porcine [y.
Specimens available in sufficient quantity were inoculated into RD and BHK cell lines.
Contract notice: Tender for the supply of iv fluids immunoglobulins and recombinant antihemophilic factor viii derived from newborn hamster kidney bhk cells.
For the supply of immunoglobulins IV fluids and recombinant antihemophilic VIII derived from newborn hamster kidney BHK cells factor.
In addition, multiple attempts to isolate this virus on various cell lines of eukaryotes (Vero E6, MDCK, CRFK, N2a, and BHK cells, primary fox kidney cells) failed because of the absence of cytopathic effects and viral replication by quantitative reverse transcription PCR, despite a high number of reads in the original sample.
In all mammalian cell types except BHK cells, we observed earlier appearance of cytopathic effects (CPE) in Kupe virus-infected cells; CPE progressed more rapidly in DUGV-infected BHK cells.
Because ITV does not produce cytopathic effects (CPE) in Vero cells, virus neutralization was conducted on BHK cells for both WNV and ITV by using WN-goose-98 and ITV (vaccine strain).
In BHK cells, a similar seroprevalence rate was observed, with titers ranging from 1:40 to 1:1,280 (3x1:40, 2x1:80, 1x1:160, 4x1:320, 3x1:640, 1x1:1280).