The water soluble carbohydrate (WSC) content was determined using an autoanalyser
according to the method used by Fulkerson et al.
Generator Set 160 KVA, AutoAnalyser
,USG Scan 3 Probe,OT table Hydraulic,Digital Video laproscopy & Haemodialysis machine to Govt Head Quarters Hospital Ariyular.
Serum concentrations of glucose, total cholesterol (TC), high density lipoprotein-cholesterol (HDL-c), triglycerides (TG), free fatty acids (FFA), homocysteine, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and fructosamine were measured in a Pentra C-200 autoanalyser
with specific colorimetric kits as described by the supplier (Horiba ABX, Montpellier, France).
1967; Kempers and Luft 1988) on an automated flow injection Skalar AutoAnalyser
Plasma glucose (PG; glucose oxidase-peroxidase) was measured on a Hitachi-912 autoanalyser
(Hitachi, Mannheim, Germany) using kits supplied by Roche Diagnostics (Mannheim, Germany).
Serum lipid levels were measured using enzymatic calorimetric method (Roche-Hitachi Modular Analytics, Tokyo, Japan) in a biochemical autoanalyser
C-reactive protein level was immunologically determined by immunoturbidimetric method (Abbott Aeroset 1600 autoanalyser
, by Abbott reagents, Germany).
Concentrations of inorganic nutrients were measured with a Skalar autoanalyser
, following the standard procedures for seawater analyses (Valderrama, 1995).
Ammonium was determined using a Technicon autoanalyser
For CCHS participants, Lp(a) concentrations were measured at the 1991-1994 examination with a well-characterized inhouse immunoturbidimetric assay using a Technicon Axon autoanalyser
(Miles), rabbit antihuman lipoprotein polyclonal antibodies (Q023, Dako), and a human serum lipoprotein calibrator (Dako) (14).
The activities of serum alkaline phosphatase (ALP) and alanine aminotransferase (ALT) and the concentrations of total protein, albumin, creatinine, and urea were determined on an autoanalyser
In the morning of the PSG monitoring, venous blood samples were taken for measurements of fasting blood glucose (hexokinase method using Autoanalyser
AU5200), triglycerides (glycerophosphate oxidase method using Autoanalyser
AU5200), and high-density lipoprotein (HDL) cholesterol (spectrophotometric method using Roche-Hitachi Modular P device).