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To better elucidate the mechanisms underlying arginine metabolism, we set out to develop a targeted metabolomics method that would simultaneously quantify 6 arginine metabolites in plasma with well-established roles in the arginine/iNOS/Arg1 pathway (see online Supplemental Fig.
We first developed an assay to measure arginine metabolism in mouse plasma and serum, and subsequently transitioned the assay to human plasma for clinical studies.
Changes of the intestinal epithelial brush border membrane arginine transport activity reflect the status of both local enterocyte's arginine metabolism as well as whole organ system arginine metabolism (Pan et al.