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ion exchange |
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1996) or multistep separation involved in heparin-affinity, anion-exchange, and metal-chelate affinity chromatography (Mostert et al. Baseline separation was achieved for all six species as well as for the internal standard (potassium hexahydroxy antimonate V) in a single chromatographic run of less than 30 min, using an ammonium carbonate buffer gradient (between 10 and 50 mM) at ambient temperature, in conjunction with cation- and anion-exchange columns in series. CSM, a highly charged quaternary, ammonium resin, may act as an absorbent by binding toxins through its strong anion-exchange capacity or by entrapment of molecules in its polymeric structure. |
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