This would contain on the order of 10 and 12 template copies (amplicons
); in other words, if a thorough cleaning reduced this by a million fold, you'd still have a million amplicon
copies "floating around," each of which could contaminate a reaction.
Fragment analysis of amplicons
was accomplished by capillary gel electrophoresis on a Beckman Coulter[TM] CEQ 8000XL Sequence Analyzer with the CEQ DNA Size Standard-400.
Contract notice: Sequencing of amplicons
taxonomic and functional sequences from soil dna on behalf of the inra research center dijon.
EvaGreen fluorescence emission from each droplet directly relates to the amount of DNA encapsulated within and will vary by amplicon
length, as has been demonstrated for 2 differently sized amplicons
from 2 targets (23)?
5 mL of rrs gene amplicons
from the first PCR amplification, we initiated the amplification protocol for the second PCR with denaturation at 94[degrees]C for 3 min, followed by 29 cycles of 94[degrees]C for 1 min, 63[degrees]C for 1.
The NEXTflex[TM] Amplicon
Panels allow researchers to sequence up to 2,000 amplicons
using a simple workflow with 100% coverage of targeted regions.
The Fluidigm Access Array System prepares amplicons
for resequencing without the need for any additional library preparation and can operate with any next-generation sequencer (NGS) on the market.
Primers should be redesigned for amplicons
that do not amplify sufficiently well despite optimization.
The authors amplified genomic material from swine sera using primers based on hepatitis E virus (HEV) genomic sequences, and sequenced the amplicons
Fluidigm's Access Array System enables automated processing of 48 unique samples against 48 unique amplicons
, resulting in PCR products which are ready for emPCR amplification and sequencing with 454 Life Sciences' GS Junior or GS FLX System.
Clear and intense amplicons
(bands) in the gel were considered to reflect that the DNA used was of high quality and quantity.
Amplification of the product specific for BYVaV was confirmed by cloning and sequencing selected amplicons