We identified 2 monoclonal antibodies that reacted with amino terminus
(26A1) and carboxy terminus (48A1) of EL among 15 clones.
DUGV and Kupe virus M segment ORFs contain a highly variable, mucin-like region near the amino terminus
, as described for the genome of CCHFV (9,11).
The enzyme can be produced with a leader peptide at its amino terminus
NIS is a protein with 13 putative transmembrane domains, an extracellular amino terminus
, and an intracellular carboxyl terminus (De La Vieja et al.
The aldolase grooves (between the A&D and B&C subunits) comprised positively charged residues (Lys's 341,348, 13,347, 27 and Arg 293) that are critical to binding with the negatively charged amino terminus
of actin (residues Asp 1,2, 3 and Glu 4) and other exposed patches of actin subdomain 1 (Asp's 24, 25, 99, 100, 364).
The Methods in Protein Structural Analysis conferences began in 1974 for the purpose of exchanging information on the newly developed automation of chemistry and technology for sequencing proteins by removing amino acids from the amino terminus
one at a time.
To protect the reporter peptides from exoproteases, the amino terminus
was blocked with a biotin-tag (25).
Direct sequencing of the RT-PCR-amplified products of a 566-nucleotide (nt) fragment coding for the amino terminus
of the nucleoprotein of EBLV and analyses of the nucleotide sequences were performed as described (7).
DNA damage induces phosphorylation of the amino terminus
Bio-intact PTH was measured by (a) a chemiluminescence immunoassay on the Nichols Advantage[R] platform (Nichols Institute Diagnostics) (2); (b) an IRMA using the Duo PTH reagents (Scantibodies Laboratory) (1); and (c) an ELISA using the Human BioActive Intact PTH reagents (Immutopics), which is a 2-site assay in which 2 goat polyclonal antibodies recognize epitopes within the midregion/carboxy terminus and at the initial amino terminus
Antibody to the amino terminus
protects animals by limiting viral replication.
It preferentially cleaves dipeptides from the amino terminus
of peptides with a proline or alanine in the second position.