After electrophoretic blotting on a nitrocellulose membrane (BA 85; Schleicher & Schuell, Dassel, Germany), the membrane was subjected to amido black staining and immunological detection to assess the effect of Es deficiency on CA.
Amido black staining resulted in appearance of smear protein bands corresponding to molecular weights of 31-43 kD and disappearance of the bands corresponding to some high-molecular-weight proteins (Fig.
Precipitated protein spots were stained either with amido black
alone or in combination with other protein stains.
9% agar gel and the results were recorded in fresh preparations and after staining with amido black.
After separation, one gel was fixed for an hour using a fixative solution (40 vol %Ethanol; 2 vol %acetic acid) and stained with amido black in order to visualise the [alpha]2M band.
Unprecipitated proteins were washed away before staining of the precipitated complexes with Amido black
Diaminobenzidine (DAB); if not available, use amido black
Diaminobenzidine (DAB); if not available, use leucocrystal violet (LCV) or amido black
Amido black is used to develop latent prints and enhance visible prints that have been deposited in blood.
The amido black process consists of two solutions--a developer and a rinse--and a final rinse of distilled water.
This amido black
water-based formula is a one-step process that includes a blood fixer.
The amido black
water-based formula is used in place of the methanol-based formula when there is a question about or a problem with a painted surface.