Moraxella catarrhalis, Haemophilus influenza, and Alloiococcus otitidis are classified as fastidious Bacteria.
Alloiococcus otitidis was first isolated from an ear fluid sample of a child in a clinical study addressing otitis media in Buffalo State, America .
The mentioned study was not capable of successfully isolating Alloiococcus otitidis of the samples with the application of culture method.
Furthermore, no instance of Alloiococcus otitidis was observed after the application of culture method.
The aim of the present study was to compare culture and Multiplex PCR methods to detect Alloiococcus otitidis, Moraxella catarrhalis, and Haemophilus influenza as three fastidious pathogens causing otitis.
Alloiococcus otitidis has small alpha-hemolytic colonies on blood agar plate, which are observable after 2-5 days of incubation.
In 50 otitis media, no cases of Alloiococcus otitidis, Moraxella catarrhalis, and Haemophilus influenza were detected using culture method.
In molecular analysis using Multiplex PCR method, no cases of the three studied bacteria were found in 50 samples of otitis externa; however, 25 (50%) samples with respect to Alloiococcus otitidis, 28 (56%) samples with respect to Haemophilus influenza, and 11 (22%) samples with respect to Moraxella catarrhalis were positive within 50 samples of otitis media (Fig.
However, recent descriptions of another possible pathogen, Alloiococcus otitidis, in significant numbers of children with AOM and otitis media with effusion in Finland, Japan, and Australia have generated debate as to the significance of this new bacterium.
Effect of Alloiococcus otitidis and three pathogens of otitis media in production of interleukin-12 by human monocyte cell line.