Alcian Blue Stain


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A proprietary water-soluble phthalocyanin dye CI 74240 used in histology, which can be modified at different pHs to identify specific mucopolysaccharide families
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At incubation intervals of 24, 48 and 72 hours, all experimental groups were stained with hematoxylin and eosin stain (H-E) and alcian blue stain.
Alcian blue stains acid mucosubstances and acetic mucins.
The Alcian blue stains were also viewed with light microscopy on 40x objective magnification.
Chondrogenic differentiation was confirmed with Alcian blue stain kit (NovaUltra[TM], IHC World, USA) by detecting glycosaminoglycan stained in blue color.
Highlighting the hyaluronic acid-rich matrix of medulloepithelioma with Alcian blue stain may aid the diagnosis of medulloepithelioma, particularly in cases that are cellular or heavily pigmented (Figure 3, inset).
The recognition of intestinal metaplasia, especially in goblet cells, is increased by the use of Alcian blue stain.
All steps were carried out by using a known ILC that expressed mucin proven by conventional Alcian blue stain on FFPE tissue.
8 We, therefore, thought of modifying the Alcian blue stain so that it could be used for SLN intraoperative diagnosis in patients with ILC.
These inclusions appeared light blue with Alcian blue stain and showed coarsely clumped staining with colloidal iron stain (Figure, C).
Alcian blue stain for acid mucopolysaccharides and periodic acid-Schiff diastase stains were negative.
The PAS and Alcian blue stains produced faint staining of the mucin between the tumor cells, but there was no convincing evidence of epithelial mucin.