AlamarBlue assay

AlamarBlue assay

An assay used to quantify the proliferation of various human and animal cell lines, bacteria and fungi, and assess relative cytotoxicity of agents within various chemical classes. The assay is based on detection of metabolic activity through an oxidation-reduction (REDOX) indicator, which both fluoresces and changes colour in response to chemical reduction of growth medium resulting from cell growth.

It is simple to perform because the indicator is water soluble, thus eliminating the washing/fixing and extraction steps required in other commonly used cell proliferation assays.