agarose gel electrophoresis


Also found in: Acronyms, Wikipedia.

Agarose Gel Electrophoresis

A technique in which large biomolecules are separated on a highly purified agarose gel by electrophoresis. AGE is used in clinical chemistry to separate mixtures of proteins by charge and size, and in molecular biology to separate mixtures of nucleic acid (DNA and RNA) fragments by sieving (movement of molecules through the gel’s pores) and size, where shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through gel pores.

agarose gel electrophoresis

see GEL ELECTROPHORESIS.
References in periodicals archive ?
coli isolates, 300 isolates were electrophorosed by agarose gel electrophoresis for detection of their resistance pattern whether it was chromosome mediated or plasmid mediated using chromosomal DNA and plasmid DNA respectively.
In figures 1B and 2B, the separation of the DNA fragments with close sizes by agarose gel electrophoresis is shown to have low resolution.
The RT-PCR products were subjected to agarose gel electrophoresis for the detection and confirmation of the rotavirus electrophoretypes.
The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining.
Agarose gel electrophoresis to check and screen the putatively positive clones containing the correct HSPa8 gene with poly-histidine tag after purification of the PCR products.
infantum by ITS1 specific primers resulted in a sharp single band on agarose gel electrophoresis.
The topics include the two-dimensional agarose gel electrophoresis of DNA topoisomers, single-molecule magnetic tweezer studies of type IB topoisomers, binding DNA topoisomers I and II to replication origins, assaying topoisomers II checkpoints in yeast, the cytological analysis of chromosome structural defects that result from topoisomers II dysfunction, and the depletion and mutation of topoisomerase II in animal cells.
Giving students the opportunity to extract, manipulate and visualise DNA molecules enhances a constructivist approach to learning about modern techniques in biology and biotechnology Visualisation usually requires agarose gel electrophoresis and staining.
Specifically, objectives were to: (1) develop a species identification key for as many Indo-Pacific Crassostrea species as possible, (2) base the key on the internal transcribed spacer (ITS-1) region of the nuclear rRNA gene as well as the cytochrome oxidase subunit I (COI) mitochondrial gene region, to provide for an internal check as well as to make the key useful in hybridization studies and for identification of hybrids in the wild, (3) Construct the key based on PCR/RFLP analyses and agarose gel electrophoresis protocols for ease of use, and (4) make the protocols and key readily available to researchers, managers, and government and other entities requiring positive identification of oyster species for purposes of restoration, management, and invasive species control.