agarose gel electrophoresis

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Agarose Gel Electrophoresis

A technique in which large biomolecules are separated on a highly purified agarose gel by electrophoresis. AGE is used in clinical chemistry to separate mixtures of proteins by charge and size, and in molecular biology to separate mixtures of nucleic acid (DNA and RNA) fragments by sieving (movement of molecules through the gel’s pores) and size, where shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through gel pores.

agarose gel electrophoresis

see GEL ELECTROPHORESIS.
References in periodicals archive ?
Rapid fish species identification by agarose gel isoelectric focusing of sarcoplasmic proteins.
Step three was to run an agarose gel electrophoresis.
He then used the molds to create leaf-surface replicas made of agarose gel.
That analogy is important, because essentially agarose gel electrophoresis starts the same way; the agarose is mixed into an aqueous solution and heated to melt, then poured into a mold to cool and form like a gelatine dessert.
visualize the PCR products in a horizontal agarose gel electrophoresis,
This quality testing level is exclusively based on molecular biology techniques such as DNA agarose gel electrophoresis, restriction digestion and analysis [19- 20] of the plasmid restriction profile along with plasmid DNA sequencing [21].
The Beckman system used a 1% agarose gel, barbital running buffer with a pH of 8.
8% Agarose gel electrophoresis and spectrophotometery procedure.
Fluid is also squeezed out onto the surface when the agarose gel is deformed, making it more challenging to generate the speckle pattern by this conventional way.
They applied agarose gel electrophoresis to samples during the baking process, which revealed that although some DNA might be degraded, it could still be possible to use PCR to detect the needed levels of DNA.
At the level of agarose gel electrophoresis, however, all individuals of the same species are indistinguishable in their ITS size.