Affinity Tag

Affinity Tag

A short peptide deliberately added to either the N- or C-end of a recombinant protein to facilitate purification of the expressed protein.
References in periodicals archive ?
The topics include mass spectrometry utilizing isotope-coded affinity tag reagents, the proteome in neurodegenerative diseases, the proteome related to respiratory disease, hepatitis C virus infection and mitochondria proteomics, and the quantitative proteomic analysis of HIV infection.
However, conventional affinity tag systems include large protein tags such as green-fluorescent proteins (9 ~ > 27kDa), FLAGs with specific antibodies (9 ~ > 150kDa), an d quantum dots (> ~ 20 [Angstrom]), (1,2) and they affect the affinity to bio-molecules.
In the first laboratory period, each pair of students receives a bacterial lysate containing a pair of unknown fluorescent proteins, one of which possesses an affinity tag.
For example, the protein samples are mixed with the ICAT reagents, which consist of a cysteine reactive moiety, a hiotin affinity tag for isolation and purification, and a linker region consisting of hydrogens (light tag) or deuteriums (heavy tag).
The first five chapters examine the production of proteins for structural biology, specifically discussing the use of a dual affinity tag for the production of recombinant protein; the cloning, expression, and purification of yeast proteins in a medium-sized structural genomics group, baculovirus expression of integral membrane proteins, and selenomethionine substitution in bacteria and eukaryotic cells.
Complementary analysis of the Mycobacterium tuberculosis proteome by two-dimensional electrophoresis and isotope coded affinity tag technology.
Oxford GlycoSciences entered a five-year collaboration to develop an industrial-scale proteomics platform using the Institute for Systems Biology's Isotope Coded Affinity Tag (ICAT) method .
This novel service is based on a revolutionary new platform, combining affinity tag binding with inert porous materials, designed especially for biocatalysis application in any organic solvent or aqueous buffer.
Chapters describe studies of protein-protein interactions using near-infrared fluorescence, nuclear magnetic resonance, and mass spectrometry; protein-ligand interactions using mass spectrometry, surface plasmon resonance, nuclear magnetic resonance, and isotope-coded affinity tag labeling; and structural proteomics using nuclear-magnetic resonance, x-ray crystallography, and electron paramagnetic resonance.
hp vectors are available for intracellular expression, without or with affinity tag for convenient purification:
His group has developed multiple methods, including isotope-coded affinity tag (ICAT) reagents for quantitative mass spectrometry and the Trans Proteomic Pipeline, a suite of software tools for the analysis of proteomic data.
Ruedi Aebersold's laboratory, has used the Isotope Coded Affinity Tag (ICAT) method and quantitative mass spectrometry to find proteins associated with these accumulated centrosomes.