Affinity Tag

Affinity Tag

A short peptide deliberately added to either the N- or C-end of a recombinant protein to facilitate purification of the expressed protein.
References in periodicals archive ?
His group has developed multiple methods, including isotope-coded affinity tag (ICAT) reagents for quantitative mass spectrometry and the Trans Proteomic Pipeline, a suite of software tools for the analysis of proteomic data.
For example, the protein samples are mixed with the ICAT reagents, which consist of a cysteine reactive moiety, a hiotin affinity tag for isolation and purification, and a linker region consisting of hydrogens (light tag) or deuteriums (heavy tag).
The first five chapters examine the production of proteins for structural biology, specifically discussing the use of a dual affinity tag for the production of recombinant protein; the cloning, expression, and purification of yeast proteins in a medium-sized structural genomics group, baculovirus expression of integral membrane proteins, and selenomethionine substitution in bacteria and eukaryotic cells.
Oxford GlycoSciences entered a five-year collaboration to develop an industrial-scale proteomics platform using the Institute for Systems Biology's Isotope Coded Affinity Tag (ICAT) method .
This novel service is based on a revolutionary new platform, combining affinity tag binding with inert porous materials, designed especially for biocatalysis application in any organic solvent or aqueous buffer.
Chapters describe studies of protein-protein interactions using near-infrared fluorescence, nuclear magnetic resonance, and mass spectrometry; protein-ligand interactions using mass spectrometry, surface plasmon resonance, nuclear magnetic resonance, and isotope-coded affinity tag labeling; and structural proteomics using nuclear-magnetic resonance, x-ray crystallography, and electron paramagnetic resonance.
hp vectors are available for intracellular expression, without or with affinity tag for convenient purification:
Ruedi Aebersold's laboratory, has used the Isotope Coded Affinity Tag (ICAT) method and quantitative mass spectrometry to find proteins associated with these accumulated centrosomes.
Jeff Ranish has used the Isotope Coded Affinity Tag (ICAT) method and quantitative mass spectrometry to find one or a few proteins that have specific properties in a complex background of other proteins that do not have this property.
of the University of Washington developed the proprietary isotope coded affinity tag technology while Dr.
The 4700 Proteomics Analyzer is expected to enable new high-throughput proteomics research and workflow approaches such as those based on the Isotope Coded Affinity Tag technology.
of the Institute for Systems Biology, developed the proprietary isotope coded affinity tag technology while he was a professor at the University of Washington.