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acid-fast stain |
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Acid-fast stain A special stain done to microscopically identify the bacteria that cause tuberculosis. Mentioned in: Sputum Culture acid-fast stain, a method of staining used in bacteriology in which a smear on a slide is treated with carbol-fuchsin stain or auramine-rhodamine stain, decolorized with acid alcohol, and counterstained with methylene blue or potassium permanganate to identify acid-fast bacteria. Acid-fast organisms resist decolorization and appear red or yellow against a dark background when viewed under a microscope. The stain may be performed on any clinical specimen but is most commonly used in examining sputum for Mycobacterium tuberculosis, an acid-fast bacillus. See also Ziehl-Neelsen test. acid-fast stain Microbiology A generic term for any of a number of special histologic stains–Ziehl-Neelsen, Kinyoun, hot carbolfuschin, et al, used to identify Mycobacterium spp How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Han bases his argument on the absence of positive acid-fast stains or
mycobacterial cultures and his assessments that the identification of M. Sputum Gram stain and acid-fast stain, sputum culture for bacteria,
sputum chlamydial antigen, throat swab for virus isolation, and urine
legionella and pneumococcal antigen tests were available for all 76
patients; all results were negative. A direct acid-fast stain showed many
acid-fast bacilli. |
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