In 41 instances the BC-GP molecular assay failed to identify any organisms, including the non-BC-GP targets such as Abiotrophia
The Indiana patient was infected with Abiotrophia (not further speciated); Granulicatella and Abiotrophia are similar bacterial genera that are difficult to distinguish by morphologic features (10).
Granulicatella and Abiotrophia species from human clinical specimens.
elegans (originally known as Abiotrophia
elegans) was first described in 1998 (1) as a catalase-negative, oxidase-negative, nonmotile, facultative anaerobic gram-positive bacterium.
The specificity of the real-time PCR assay was verified by testing genomic DNA from bacterial species that are phylogenetically close to GBS, including members of the genera Streptococcus, Lactococcus, Enterococcus, Abiotrophia, Peptostreptococcus, and Listeria.
Gram-positive aerobic bacteria Abiotrophia adiacens ATCC 49175 (a) A.
To the Editor: Granulicatella and Abiotrophia, formerly known as nutritionally variant streptococci, are normal flora of the human upper respiratory, gastrointestinal, and urogenital tracts (1).
16S rRNA gene sequencing may facilitate or confirm Granulicatella and Abiotrophia spp.
99 Eubacterium brachy 99 Gemella haemolysans 99 Mycoplasma orale 95 Comamonas denitrificans 95 Prevotella tannerae 98 Peptostreptococcus micros 99 209 Abiotrophia
defectiva 99 Acidobacteria sp.
Results of culture and PCR assays of 786 biopsy lymph node specimens * 16S Bartonella- rDNA- Diagnosis or Positive positive positive infection culture PCR PCR Total CSD 1 244 122 244 Bartonella quintana 1 1 1 1 Q fever 2 0 3 3 Tularemia 1 0 1 1 Abiotrophia
2 0 2 2 adjacens Actinomyces 1 0 1 1 Pasteurella 2 0 2 2 multocida Mycobacterial 54 0 32 54 infection Staphylococcus 16 0 16 16 aureus Coagulase- 15 0 10 23 negative Staphylococcus Streptococcus 10 0 10 10 pyogenes Fusobacterium spp.
rpoB gene sequence-based identification of aerobic gram-positive cocci of the genera Streptococcus, Enterococcus, Gemella, Abiotrophia
, and Granulicatella.
Thus, the isolation of fastidious microorganisms including Abiotrophia
(new genus Granulicatella) and the HACEK group, has improved dramatically, and organisms frequently missed with the use of earlier blood culture techniques are now recognized.