dark red coloured with a transparent border, 'Bulls-eye') are then further identified using the biochemical identification systems API 10S and/or API 20E
standardised identification system (Biomerieux).
However, FE109046 showed biochemical reactions in tubes and when using API 20E
(bioMerieux, Durham, NC, USA) that were identical to reactions for isolate FE109024 after an overnight and 3-day incubation (online Technical Appendix Table).
All test tubes that were urease negative were treated as suspect Salmonella and confirmed by using API 20E
Diluent composition for use of API 20E
in characterizing marine and estuarine bacteria.
For selective plating, presumptive Salmonella colonies from selective plates were confirmed with the API 20E
identification system (BioMerieux).
Using the API 20E
test kit, we isolated Staphylococcus aureus and a gram-negative bacillus similar to Mannheimia haemolytica (67%) or Morganella morganii (26%).
5 mg/L meropenem to ensure culture purity; colonies were identified by using API 20E
strips (bioMerieux, Basingstoke, UK).
Further biochemical characterization with the API 20E
panel indicated that the isolates produced lysine decarboxylase and ornithine decarboxylase and fermented D-glucose, D-mannitol, and Larabinose.
sonnei by the API 20E
strip (bioMerieux Italia, Florence, Italy).
Yellow colonies from TCBS agar were tested by using MicroScan Walk-Away 96 (Dade Behring, West Sacramento, CA, USA) panel NBPC 34 and API 20E
strip (bioMerieux, Marcy l'Etoile, France), which suggested V.
The API 20E
system (bioMerieux, Marcy-l'Etoile, France) according to the manufacturer's instructions without C[O.
API 20NE, API 20E
, and VITEK 2 Auto system (bio-Merieux, Marcy l'Etoile, France) did not identify the bacillus.