ANXA5

ANXA5

A gene on chromosome 4q27 that encodes annexin 5, a calcium-dependent phospholipid-binding protein that inhibits phospholipase A2 and protein kinase C kinase, has anticoagulant activity, and may have a role in cell signal transduction, inflammation, growth and differentiation.
References in periodicals archive ?
Ca], we also identified ANXA5 as a potential interacting protein of [BK.
The aim of this study was to validate the interaction of ANXA5 and [BK.
Knockdown and overexpression of ANXA5 were achieved by transient transfection of cells with specific siRNA and ANXA5 plasmid DNA, respectively.
Ca][alpha] currents were recorded from inside-out membrane patches excised from the stably expressing the wild type HEK293 cells, transiently transfected with the ANXA5 siRNA or the ANXA5 plasmid.
Ca] cells transfected with ANXA5 siRNA (see above) were plated into 96-well white walled plates, in triplicate for each experimental condition.
Ca][alpha] (Alamone) or ANXA5 (Santa Cruz) antibody was added.
Although binding of ANXA5 to phosphatidylserine that results in its exposure to the external leaflet of the plasma membrane is routinely used as an assay of cell death, little information is available on the potential role of annexins in the earlier stages of apoptosis.
Knockdown of ANXA5 using siRNA (confirmed by the immunocytochemistry shown in Figure 2(c)) resulted in a significant leftward shift of [Ca.
2+] was effectively modulated by ANXA5 expression was also approved by the observation that the control [BK.
Here we identify ANXA5 as a protein which physically communicates with [BK.
Therefore, the data of this study show that the physical partnership of ANXA5 and [BK.
Caption: Figure 1: Serum deprivation-evoked apoptosis results in early localization of ANXA5 to plasma membrane outer leaflet.