After sonication, 5 [micro]g of antibodies recognizing the following AML1 (Santa Cruz Biotechnology, Santa Cruz, USA, sc-28679), ETO (Santa Cruz Biotechnology, Santa Cruz, USA, sc-9737), histone deacetylase 1 (HDAC1) (Abcam, Cambridge, USA, ab7028), DNA methyltransferase 1 (DNMT1) (Abcam, Cambridge, USA, ab13537), DNMT3a (Abcam, Cambridge, USA, ab13888) and DNMT3b (Abcam, Cambridge, USA, ab13604) were immunoprecipitated with the chromatin overnight.
We then observed that the expression of AML1-ETO could cause a dose-dependent decrease in the luciferase reporter activity of the EYA4-P1, but not in its mutant EYA4-M and EYA4-P2 that lack any functional AML1 binding site [Figure 2]b.
Therefore, the chromatin remodeling complex which was aberrantly formed by AML1-ETO, and the hypermethylation of the CpG islands which were presented in the AML1 binding sites on the EYA4 regulatory region appeared to be the key regulatory mechanisms for the transcriptional silencing of EYA4 .
sup],, All reported cases with ZNF198-FGFR1 in blastic phase at diagnosis showed confirmative phenotypic alterations of neoplastic cells/blasts derived from B or T cell lineage with or without CD34, and the occurrence of hypereosinophilia in blood and marrow of patients with AML was usually found in association with CBF[sz]-MYH11 fusion gene in AML-M4Eo with inv (16); t (16;16) or in a minority of patients with a AML1
- ETO fusion gene in AML-M2 with a t (8;21).
Recently, Fontana et al (7) have shown the inhibitory role of miRNA 17-5P-20a-106a in monocytopoiesis through targeting AML1
(Figure 3), a subunit of core binding factor, leading to downregulation of macrophage colony-stimulating factor receptor.