ADH1B

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ADH1B

A gene on chromosome 4q21-23 that encodes a member of the alcohol dehydrogenase family, which metabolise various substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids and lipid peroxidation products. The encoded protein plays a major role in ethanol catabolism.
References in periodicals archive ?
Genotyping of human alcohol dehydrogenases at the ADH2 and ADH3 loci following DNA sequence amplification.
Isoform Restriction (primer ID) Primer sequence enzyme ALDH2 (YC3) 5'-TTG GTG GCT AGA AGA TGT C-3' MboII ALDH2 (YC4) 5'-CCA CAC TCA CAG TTT TCT CTT-3' MboII ADH2 (A2F) 5'-ATT CTA AAT TGT TTA ATT CAA GAA G-3' MsII ADH2 (A2R) 5'-ACT AAC ACA GAA TTA CTG GAC-3' MsII ADH2 (424) 5'-TGG ACT CTC ACA ACA AGC ATG GT-3' AluI ADH2 (290) 5'-TTT CTT TGG AAA GCC CCC AT-3' AluI ADH2 (352) 5'-TCT TTC CTA TTG CAG TAG C-3' AluI ADH3 (321) 5'-GCT TTA AGA GTA AAT ATT CTG TCC CC-3' SspI ADH3 (351) 5'-AAT CTA CCT CTT TCC GAA GC-3' SspI Table 2.
The ADH2 genotype was determined by PCR and subsequent digestion with MaeIII, according to the method of Xu et al.
2] groups, according to the ADH2 genotypes, there were no significant differences in age, body mass index, lifestyle habits, or frequency of the ALDH genotypes.
The associations between the ADH2 or ALDH2 genotype and the risk factors for CHD were further assessed by multiple logistic regression analysis (Table 3).
The present study indicates that the ADH2 genotype, but not the ALDH2 genotype, might be involved in individual variations in blood pressure, triglycerides, and uric acid in relation to alcohol consumption.
After amplification of the ADH2 allele, 2 [micro]L of the PCR products was used for the SSCP analysis, and 20 [micro]L of the PCR products was precipitated with ethanol.
The mobilities of the PCR products from three ADH2 genotypes ([ADH2.
These results demonstrate that ADH2 genotyping can be carried out successfully by SSCP analysis.
In conclusion, (a) nucleic acids were successfully extracted from nail clippings by the present guanidine method, which was improved from the previous one that had succeeded DNA extraction even from manicured or smudgy nails without contamination due to using a nail clipper [12], (b) the ADH2 genotype was detected with complete specificity by the present method, and (c) nails may be a suitable material for DNA analysis with PCR in a population study, not least through ease in obtaining informed consent from the study subjects.
Improved method for genotype determination of human alcohol dehydrogenase (ADH) at ADH2 and ADH3 loci by using polymerase chain reaction-directed mutagenesis.
A group of seven such well-designed studies focused on the association of the ADH2 and ADH3 genes with alcoholism.