8-OHdG

8-OHdG

8-hydroxy-2’-deoxyguanosine. A modified DNA nucleoside product generated by reactive oxygen species (ROS), which is elevated in colorectal carcinoma, but not in adenomas. 8-OHdG is also elevated in ulcerative colitis (UC) and increases with disease duration and dysplasia. MUTYH, the human mutY homologue, is responsible for excising misincorporated adenine and for suppressing mutations caused by ROS. Nuclear expression of MUTYH is attenuated in UC.
References in periodicals archive ?
Elevated serum 8-OHdG is associated with poor prognosis in epithelial ovarian cancer.
Artesunate suppressed cigarette smoke-induced increases in BAL fluid total and differential cell counts; levels of IL-1 p, MCP-1, IP-10 and KC; and levels of oxidative biomarkers 8-isoprostane, 8-OHdG and 3-nitrotyrosine in a dose-dependent manner.
5 ounces) for six months lowered 8-isoprostane and 8-OHdG concentrations, both markers of oxidative stress.
8-OHdG can be incorporated into DNA regions rich in the nucleotides cytosine and guanosine (i.
8-OHdG is a conventional model to demonstrate ROS-induced DNA damage, because this modified base is important in mutagenesis and carcinogenesis [6].
In animal models, tea polyphenols have been shown to inhibit carcinogen-induced increases in urinary 8-OHdG.
Lymphocyte DNA fragmentation (Comet Assay) and 24h urinary 8-OHdG excretion were measured to determine DNA damage.
Plasma levels of 8-OHdG were measured at 450 nm on a microplate plate reader using a commercial kit from the Japan Institute for the Control of Aging (Fukuroi, Japan).
8-OHdG is a marker of oxidative stress in chronic inflammatory disease.
In another study, concentrations of organochlorines, 8-OHdG (8-hydroxy-2-deoxyguanosine, a biomarker of oxidative DNA damage), DDT, and DDE were measured in 44 cancerous and 21 noncancerous breast tissues (Charles et al.