The pathway can be considered in two phases: the irreversible oxidative phase comprising the reactions catalyzed by glucose 6-phosphate dehydrogenase, lactonase and 6-phosphogluconate dehydrogenase
; and the reversible, non-oxidative phase involving the rest of the pathway, then: Regulation of the pentose phosphate pathway: The flow of metabolites through the pathway is regulated at the glucose 6-phosphate dehydrogenase reaction and the 6-phosphogluconate dehydrogenase
by the availability of NADP +.
System 9 was used to resolve malate dehydrogenase (Mdh-1, Mdh-2, Mdh-3, Mdh-4), 6-phosphogluconate dehydrogenase
(6-Pgd-1), and phosphoglucomutase (Pgm-1, Pgm-2).
The two strains differ by the mobility of a single isoenzyme 6-phosphogluconate dehydrogenase
(6PGDH) as detected by isoenzyme characterization (15), which is considered as the gold standard in species diagnosis.
In Gelditsia triacanthos, isozyme-2 of 6-phosphogluconate dehydrogenase locus is an accurate predictor of sex in 5 natural populations.
1990)" Non --random association between sex and 6-phosphogluconate dehydrogenase isozyme genotypes in Gelditsia triacanthos L".
The 6-phosphogluconate dehydrogenase
(6PGDH) gene was chosen because it shows a high degree of sequence polymorphism among Leishmania species (12), is well represented in sequence databases, and is known to differentiate the main zymodeme from L.
2 PGM-1* M,H,L PGM-2* H 6-Phosphogluconate dehydrogenase
MDHP), peroxidase (PER), 6-phosphogluconate dehydrogenase
Simultaneous automated determination of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase
activities in whole blood.
Of the enzyme systems initially assayed, alcohol dehydrogenase (ADH); lactate dehydrogenase (LDH); malate dehydrogenase (MDH); phosphohexose isomerase (PHI/PGI); and 6-phosphogluconate dehydrogenase
(6-PGD) resolved consistently.
The enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase
preserve glucose metabolism at a cost to ribose synthesis.
The Beutler enzyme spot test utilizes the phosphoglucomutase, G6PD, and 6-phosphogluconate dehydrogenase
present naturally in RBCs as the enzyme reactions subsequent to the GALT enzyme.