The ability of CD to convert nontoxic 5FC into toxic 5FU antimetabolite and its absence in mammalian cells has been exploited in enzyme-prodrug gene therapy and shown to control tumour growth (Hamaji et al.
coli because it displays a kinetic advantage towards 5FC over E.
The enzyme from either of these sources has limitations because yeast CD has a higher affinity for 5FC than E.
It was thus unexpected to observe 35% resistance to 5FC among C.
For nonclinical isolates, only MICs of 5FC and fluconazole were determined.
In light of these results, susceptibility to 5FC ([sub.
tropicalis synonyms stored at the CBS exhibited 5FC MICs [less than or equal to] 0.
We then studied genes potentially involved in the mechanisms of 5FC resistance.
tropicalis isolates resistant to 5FC is unusual (3).
albicans isolates with decreased susceptibility to 5FC, remains to be determined (21).
The resistance to 5FC was associated with the K177E mutation in the URA3 gene in the clone.