Eriksson, "Ethanol, acetaldehyde, acetate, and lactate levels after alcohol intake in white men and women: effect of 4-methylpyrazole
," Alcoholism: Clinical and Experimental Research, vol.
The customary antidote for dogs at Cornell is 4-methylpyrazole
, also known as fomepizole, or if that is not available, ethanol, Dr.
The treatments were a urease inhibitor (Agrotain; N-(n-butyl) thiophosphoric triamide), 2 nitrification inhibitors (dicyandiamide, DCD; 4-methylpyrazole, 4MP), a combination (DCD+Agrotain), a urine control, and a nil urine.
There were 6 treatments: a urease inhibitor (Agrotain) with cow urine; 2 nitrification inhibitors (DCD and 4-methylpyrazole, 4MP) mixed separately with cow urine; a urease inhibitor plus a nitrification inhibitor (Agrotain+DCD) mixed together in cow urine; and 2 controls (nil urine and urine control without inhibitors).
Use of 4-methylpyrazole
was also greater in the inhalation group (24%) than in the ingestion group (20%).
4-methylpyrazole, have been used to treat ethylene glycol poisoning in humans [69,70].
Treatment of ethylene glycol poisoning with intravenous 4-methylpyrazole.
Efficacy of 4-methylpyrazole in ethylene glycol poisoning: clinical and toxicokinetic aspects.
HRP-conjugated antibodies to c-myc tag (18824P) were from QED Bioscience (San Diego, CA, USA), primary antibodies to cytochrome P4502E1 (ab28146), [beta]-actin (ab3280), HCV core (ab58713), and HRP-conjugated anti-rabbit and anti-mouse secondary antibodies, as well as CYP2E1 inhibitor 4-methylpyrazole were purchased from Abcam (Cambridge, UK).
In addition, in some experiments, the cells were treated with 100 [micro]M 4-methylpyrazole (4-MP), 100 [micro]M BAPTA-AM, or 100 [micro]M Ru360.
Compounds other than alcohol also are very good inducers of CYP2E1, including 4-methylpyrazole
, a drug now used for the treatment of methanol and ethylene glycol poisoning.