One molecule, 3-hydroxyanthranilic acid, was orders of magnitude better than the others, and was selected for follow-up in the lab.
Weaver wants to study the brains of deceased people with and without Alzheimer's to see if there's a difference in 3-hydroxyanthranilic acid concentration, as well as to study the pharmacokinetics of this molecule and its derivatives.
A computerized screen of chemicals found in the brain has identified 3-hydroxyanthranilic acid as a potential Alzheimer's therapeutic.
Folate (5-methyltetrahydrofolate), riboflavin, flavin mononucleotide (FMN),  FAD, pyridoxal 5'-phosphate (PLP), pyridoxal (PL), pyridoxic acid, free choline, betaine, dimethylglycine, creatinine, methionine sulfoxide, arginine, asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA), tryptophan, kynurenine, kynurenic acid, anthranilic acid, 3-hydroxykynurenine, xanthurenic acid, 3-hydroxyanthranilic acid, and formiminoglutamic acid, were determined by liquid chromatography--tandem mass spectrometry (14-17), and methylmalonic acid, total homocysteine, total cysteine, methionine, serine, glycine, cystathionine, and sarcosine by gas chromatography-tandem mass spectrometry (15, 18).
The most pronounced declines (92%-12%) in serum concentrations during storage at room temperature or in frozen samples were observed for FMN (not analyzed at -25 [degrees]C), folate (5-methyltetrahydrofolate), PLP, 3-hydroxykynurenine, and 3-hydroxyanthranilic acid, and the most pronounced increases (1700%-19%) were observed for choline, PL, riboflavin, serine, and arginine (Fig.
In frozen serum samples, 3-hydroxyanthranilic acid ([DT.