This culture media consists of methyl cellulose, FBS, BSA, 2-mercaptoethanol
, L-glutamine, recombinant human (rh) stem cell factor, rh granulocyte-macrophage colony-stimulating factor (GM-CSF), rh interleukin (IL)-3, rhlL-6, rhG-CSF, and rhErythropoietin.
5M Urea, 4% NP-40, 5% (v/v) 2-mercaptoethanol
, 2% v/v ampholytes of pH 5-8 and pH 3-10.
After addition of 2-mercaptoethanol
(2%), samples were boiled for 5 min and used for the experiments.
In the presence of sodium dodecyl sulphate and 2-mercaptoethanol
, the E7 protein migrated as a 23kDa protein during poly acrylamide gel electrophoresis.
0) and 30 pi of 2-mercaptoethanol
were added) and then injected into the chromatographic system.
2), 100 mM Na3F, 5 mM EDTA, 5 mM EGTA, and 15 mM 2-mercaptoethanol
restores both activity and normal electrophoretic migration, suggesting a disulfide bond.
The Wright agglutination test and 2-mercaptoethanol
test for brucella were positive at titers of 1/320 and 1/160, respectively.
1 mM 2-mercaptoethanol
, 100 units of penicillin/ml, and 100 [micro]g of streptomycin/ml (Meyer et al.
Gelotte and Joseph D'Silva Assigned: Aventis Pharmaceuticals Products Keywords: Exposing EPDM to 2-mercaptoethanol
5] mol/L 2-mercaptoethanol
(Gibco), antibiotic-antimycotic solution (100 U/mL penicillin, 100 [micro]g/mL streptomycin, and 0.
Although solubilization of proteins is more easily obtained by the use of 2-mercaptoethanol
, this solution is used to dissolve sulphydryl bonds.