2-mercaptoethanol


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2-mer·cap·to·eth·a·nol

(mer-kap'tō-eth'ă-nol),
A reagent (HOCH2CH2SH) used to reduce disulfide bonds, particularly in proteins, and to prevent their formation.
Synonym(s): β-mercaptoethanol

mercaptoethanol

A reducing reagent which contains a thiol group that breaks disulfide bonds in proteins, rendering them functionally effete.
References in periodicals archive ?
Following our previous work (24), [beta]-elimination with a mild alkylamine base and concomitant Michael-type addition of 2-mercaptoethanol as nucleophile was applied.
5,8] The serum samples were further tested with 2-mercaptoethanol (2-ME) to confirm acute brucellosis infection.
Fifty mg of each tissue in RNAlater were homogenized in RTL lysis buffer (Qiagen) containing 1% 2-mercaptoethanol.
3) Protease inhibitors stops proteolytic cleavage of antibody while reducing agents like dithiothreitol (DTT) and 2-mercaptoethanol (2-ME) also prevents oxidation in the conjugate.
The digested tissue was removed to a 6-cm dish flooded with essential culture medium (alpha-minimum essential medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 100 [micro]g/mL Streptomycin, 2 mM L-glutamine, 5 x 10[sup]−5 M 2-mercaptoethanol and 10[sup]−4 M L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate).
The cells was lysed with sampling buffer (2% SDS, 5% 2-mercaptoethanol, 10% glycerol, and 0.
Sample buffer, with 5% 2-mercaptoethanol, was added to 40 mg protein per lane and boiled for 2 min.
HEPES, penicillin, streptomycin, sodium bicarbonate, 2-mercaptoethanol (2-ME), bovine serum albumin (BSA), histopaque, fetal calf serum (FCS), RPMI 1640 medium, M 199 medium were purchased from Sigma-Aldrich.
19) On the other hand, on the slab SDS-PAGE in the absence of 2-mercaptoethanol the purified enzyme showed two spots, 106 and 95kDa, both of which showed an identical amino acid composition and peptide mapping.

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