The 21-OH enzyme converts 17-hydroxyprogesterone to 11-deoxycortisol
and progesterone to deoxycorticosterone, which are precursors for cortisol and aldosterone synthesis.
Metyrapone reversibly inhibits 11-[beta] hydroxylase, preventing oxidation of 11-deoxycortisol
One of the enzymes affected is 11-beta-hydroxylase , which is responsible for the conversion of 11-desoxycorticosterone to corticosterone (both hormones possessing mineralocorticoid activity and being precursors to aldosterone) and 11-deoxycortisol
to cortisol (10).
The exact diagnosis of 11-[beta]-hydroxylase deficiency can be performed by the high basal levels of deoxycorticosterone and or 11-deoxycortisol
serums or tetrahydrometabolites, which might be found during a 24-hour urine test.
17-OHP is then converted to 11-deoxycortisol
DISCUSSION: Defective conversion of 17-hydroxyprogesterone to 11-deoxycortisol
accounts for more than 95% of cases of congenital adrenal hyperplasia.
All core clinical biochemistry and hormonal tests were performed at Royal Hospital, Oman, using Architect 8000c and Architect 2000i, respectively, with Automated Processing System (Abbott, USA); and 17[alpha]-hydroxyprogesterone, renin and aldosterone were performed by ELISA (DRG, USA); while 11-deoxycortisol
, 11-deoxycorticosterone, corticosterone, androstenedione and ACTH were performed at Laboratoroire Cerba (France).
Improved HPLC method for simultaneous analysis of cortisol, 11-deoxycortisol
, prednisolone, methylprednisolone, and dexamethasone in serum and urine.
Weiming Li, professor of fisheries and wildlife who helped lead the project, said: "By identifying 11-deoxycortisol
as a stress hormone in lamprey, it allows us to better understand how the endocrine system in vertebrates evolved into the complex systems we see in humans today.
Steroid concentrations in the medium and cell lysate were measured using liquid chromatography/mass spectrometry for 12 steroids [pregnenolone (PREG), 17[alpha]-hydroxypregnenolone (HPREG), dehy-droepiandrosterone (DHEA), progesterone (PROG), 17[alpha]-hydroxyprogesterone (HPROG), androstenedione (DIONE), testosterone (T), deoxycorticosterone (DCORTICO), corticos-terone (CORTICO), ALDO, 11-deoxycortisol
(DCORT), and CORT] and using enzyme-linked immunosorbent assay for two additional steroids [estrone ([E.
To eliminate endocrinological causes of hypertrichosis, serum levels of testosterone, DHEA-SO4, 17-OH progesterone, and 11-deoxycortisol
were measured and were found to be within normal limits.
We measured 15 adrenal steroids simultaneously by LCMS/MS, including aldosterone, cortisol, 18-oxocortisol, 18-hydroxycortisol, cortisone, 11-deoxycortisol
, 21deoxycortisol, corticosterone, 11-deoxycorticosterone, progesterone, 17-hydroxyprogesterone, pregnenolone, androstenedione, dehydroepiandrosterone (DHEA), and DHEA-sulfate (DHEA-S).