1-fluoro-2,4-dinitrobenzene


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1-fluor·o-2,4-di·ni·tro·ben·zene (FDNB),

(flōr'ō-dī-nī'trō-ben'zēn),
A reagent used to combine with the free amino groups of aminoacyl residues in a peptide, thus marking those residues; the combined forms are known as DNP-proteins, Dnp-aminoacyl amino acids, the fluorine having been replaced to leave a dinitrophenyl residue (DNP, Dnp, or N2Ph-) attached to the NH2 group. Hence, the N-terminal amino acid and lysine side chains will be covalently modified.
Synonym(s): Sanger reagent
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